Isolation and characterization of lignin-degrading bacteria from rainforest soils.
Identifieur interne : 002614 ( Main/Exploration ); précédent : 002613; suivant : 002615Isolation and characterization of lignin-degrading bacteria from rainforest soils.
Auteurs : Xing-Feng Huang [États-Unis] ; Navaneetha Santhanam ; Dayakar V. Badri ; William J. Hunter ; Daniel K. Manter ; Stephen R. Decker ; Jorge M. Vivanco ; Kenneth F. ReardonSource :
- Biotechnology and bioengineering [ 1097-0290 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), ADN bactérien (génétique), ARN ribosomique 16S (génétique), Arbres (MeSH), Bactéries (enzymologie), Bactéries (génétique), Bactéries (isolement et purification), Bactéries (métabolisme), Biocarburants (MeSH), Biomasse (MeSH), Laccase (génétique), Lignine (analyse), Lignine (composition chimique), Lignine (métabolisme), Microbiologie du sol (MeSH), Populus (MeSH), Protéines bactériennes (génétique), Pérou (MeSH), Écosystème (MeSH).
- MESH :
- analyse : ADN bactérien, Lignine.
- composition chimique : Lignine.
- enzymologie : Bactéries.
- génétique : ADN bactérien, ARN ribosomique 16S, Bactéries, Laccase, Protéines bactériennes.
- isolement et purification : Bactéries.
- métabolisme : Bactéries, Lignine.
- Arbres, Biocarburants, Biomasse, Microbiologie du sol, Populus, Pérou, Écosystème.
- Wicri :
- geographic : Pérou.
English descriptors
- KwdEn :
- Bacteria (enzymology), Bacteria (genetics), Bacteria (isolation & purification), Bacteria (metabolism), Bacterial Proteins (genetics), Biofuels (MeSH), Biomass (MeSH), DNA, Bacterial (analysis), DNA, Bacterial (genetics), Ecosystem (MeSH), Laccase (genetics), Lignin (analysis), Lignin (chemistry), Lignin (metabolism), Peru (MeSH), Populus (MeSH), RNA, Ribosomal, 16S (genetics), Soil Microbiology (MeSH), Trees (MeSH).
- MESH :
- chemical , analysis : DNA, Bacterial, Lignin.
- chemical , chemistry : Lignin.
- chemical , genetics : Bacterial Proteins, DNA, Bacterial, Laccase, RNA, Ribosomal, 16S.
- geographic : Peru.
- enzymology : Bacteria.
- genetics : Bacteria.
- isolation & purification : Bacteria.
- metabolism : Bacteria, Lignin.
- chemical : Biofuels, Biomass, Ecosystem, Populus, Soil Microbiology, Trees.
Abstract
The deconstruction of lignin to enhance the release of fermentable sugars from plant cell walls presents a challenge for biofuels production from lignocellulosic biomass. The discovery of novel lignin-degrading enzymes from bacteria could provide advantages over fungal enzymes in terms of their production and relative ease of protein engineering. In this study, 140 bacterial strains isolated from soils of a biodiversity-rich rainforest in Peru were screened based on their oxidative activity on ABTS, a laccase substrate. Strain C6 (Bacillus pumilus) and strain B7 (Bacillus atrophaeus) were selected for their high laccase activity and identified by 16S rDNA analysis. Strains B7 and C6 degraded fragments of Kraft lignin and the lignin model dimer guaiacylglycerol-β-guaiacyl ether, the most abundant linkage in lignin. Finally, LC-MS analysis of incubations of strains B7 and C6 with poplar biomass in rich and minimal media revealed that a higher number of compounds were released in the minimal medium than in the rich one. These findings provide important evidence that bacterial enzymes can degrade and/or modify lignin and contribute to the release of fermentable sugars from lignocellulose.
DOI: 10.1002/bit.24833
PubMed: 23297115
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Badri</name></author><author><name sortKey="Hunter, William J" sort="Hunter, William J" uniqKey="Hunter W" first="William J" last="Hunter">William J. Hunter</name></author><author><name sortKey="Manter, Daniel K" sort="Manter, Daniel K" uniqKey="Manter D" first="Daniel K" last="Manter">Daniel K. Manter</name></author><author><name sortKey="Decker, Stephen R" sort="Decker, Stephen R" uniqKey="Decker S" first="Stephen R" last="Decker">Stephen R. Decker</name></author><author><name sortKey="Vivanco, Jorge M" sort="Vivanco, Jorge M" uniqKey="Vivanco J" first="Jorge M" last="Vivanco">Jorge M. Vivanco</name></author><author><name sortKey="Reardon, Kenneth F" sort="Reardon, Kenneth F" uniqKey="Reardon K" first="Kenneth F" last="Reardon">Kenneth F. 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Badri</name></author><author><name sortKey="Hunter, William J" sort="Hunter, William J" uniqKey="Hunter W" first="William J" last="Hunter">William J. Hunter</name></author><author><name sortKey="Manter, Daniel K" sort="Manter, Daniel K" uniqKey="Manter D" first="Daniel K" last="Manter">Daniel K. Manter</name></author><author><name sortKey="Decker, Stephen R" sort="Decker, Stephen R" uniqKey="Decker S" first="Stephen R" last="Decker">Stephen R. Decker</name></author><author><name sortKey="Vivanco, Jorge M" sort="Vivanco, Jorge M" uniqKey="Vivanco J" first="Jorge M" last="Vivanco">Jorge M. Vivanco</name></author><author><name sortKey="Reardon, Kenneth F" sort="Reardon, Kenneth F" uniqKey="Reardon K" first="Kenneth F" last="Reardon">Kenneth F. Reardon</name></author></analytic><series><title level="j">Biotechnology and bioengineering</title><idno type="eISSN">1097-0290</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (enzymology)</term><term>Bacteria (genetics)</term><term>Bacteria (isolation & purification)</term><term>Bacteria (metabolism)</term><term>Bacterial Proteins (genetics)</term><term>Biofuels (MeSH)</term><term>Biomass (MeSH)</term><term>DNA, Bacterial (analysis)</term><term>DNA, Bacterial (genetics)</term><term>Ecosystem (MeSH)</term><term>Laccase (genetics)</term><term>Lignin (analysis)</term><term>Lignin (chemistry)</term><term>Lignin (metabolism)</term><term>Peru (MeSH)</term><term>Populus (MeSH)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Soil Microbiology (MeSH)</term><term>Trees (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (analyse)</term><term>ADN bactérien (génétique)</term><term>ARN ribosomique 16S (génétique)</term><term>Arbres (MeSH)</term><term>Bactéries (enzymologie)</term><term>Bactéries (génétique)</term><term>Bactéries (isolement et purification)</term><term>Bactéries (métabolisme)</term><term>Biocarburants (MeSH)</term><term>Biomasse (MeSH)</term><term>Laccase (génétique)</term><term>Lignine (analyse)</term><term>Lignine (composition chimique)</term><term>Lignine (métabolisme)</term><term>Microbiologie du sol (MeSH)</term><term>Populus (MeSH)</term><term>Protéines bactériennes (génétique)</term><term>Pérou (MeSH)</term><term>Écosystème (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>DNA, Bacterial</term><term>Laccase</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Peru</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN bactérien</term><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>ARN ribosomique 16S</term><term>Bactéries</term><term>Laccase</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacteria</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bactéries</term><term>Lignine</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biofuels</term><term>Biomass</term><term>Ecosystem</term><term>Populus</term><term>Soil Microbiology</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arbres</term><term>Biocarburants</term><term>Biomasse</term><term>Microbiologie du sol</term><term>Populus</term><term>Pérou</term><term>Écosystème</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Pérou</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The deconstruction of lignin to enhance the release of fermentable sugars from plant cell walls presents a challenge for biofuels production from lignocellulosic biomass. The discovery of novel lignin-degrading enzymes from bacteria could provide advantages over fungal enzymes in terms of their production and relative ease of protein engineering. In this study, 140 bacterial strains isolated from soils of a biodiversity-rich rainforest in Peru were screened based on their oxidative activity on ABTS, a laccase substrate. Strain C6 (Bacillus pumilus) and strain B7 (Bacillus atrophaeus) were selected for their high laccase activity and identified by 16S rDNA analysis. Strains B7 and C6 degraded fragments of Kraft lignin and the lignin model dimer guaiacylglycerol-β-guaiacyl ether, the most abundant linkage in lignin. Finally, LC-MS analysis of incubations of strains B7 and C6 with poplar biomass in rich and minimal media revealed that a higher number of compounds were released in the minimal medium than in the rich one. These findings provide important evidence that bacterial enzymes can degrade and/or modify lignin and contribute to the release of fermentable sugars from lignocellulose.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23297115</PMID><DateCompleted><Year>2013</Year><Month>10</Month><Day>24</Day></DateCompleted><DateRevised><Year>2013</Year><Month>04</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1097-0290</ISSN><JournalIssue CitedMedium="Internet"><Volume>110</Volume><Issue>6</Issue><PubDate><Year>2013</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Biotechnology and bioengineering</Title><ISOAbbreviation>Biotechnol Bioeng</ISOAbbreviation></Journal><ArticleTitle>Isolation and characterization of lignin-degrading bacteria from rainforest soils.</ArticleTitle><Pagination><MedlinePgn>1616-26</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/bit.24833</ELocationID><Abstract><AbstractText>The deconstruction of lignin to enhance the release of fermentable sugars from plant cell walls presents a challenge for biofuels production from lignocellulosic biomass. The discovery of novel lignin-degrading enzymes from bacteria could provide advantages over fungal enzymes in terms of their production and relative ease of protein engineering. In this study, 140 bacterial strains isolated from soils of a biodiversity-rich rainforest in Peru were screened based on their oxidative activity on ABTS, a laccase substrate. Strain C6 (Bacillus pumilus) and strain B7 (Bacillus atrophaeus) were selected for their high laccase activity and identified by 16S rDNA analysis. Strains B7 and C6 degraded fragments of Kraft lignin and the lignin model dimer guaiacylglycerol-β-guaiacyl ether, the most abundant linkage in lignin. Finally, LC-MS analysis of incubations of strains B7 and C6 with poplar biomass in rich and minimal media revealed that a higher number of compounds were released in the minimal medium than in the rich one. These findings provide important evidence that bacterial enzymes can degrade and/or modify lignin and contribute to the release of fermentable sugars from lignocellulose.</AbstractText><CopyrightInformation>Copyright © 2013 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Xing-Feng</ForeName><Initials>XF</Initials><AffiliationInfo><Affiliation>Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523-1370, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Santhanam</LastName><ForeName>Navaneetha</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Badri</LastName><ForeName>Dayakar V</ForeName><Initials>DV</Initials></Author><Author ValidYN="Y"><LastName>Hunter</LastName><ForeName>William J</ForeName><Initials>WJ</Initials></Author><Author ValidYN="Y"><LastName>Manter</LastName><ForeName>Daniel K</ForeName><Initials>DK</Initials></Author><Author ValidYN="Y"><LastName>Decker</LastName><ForeName>Stephen R</ForeName><Initials>SR</Initials></Author><Author ValidYN="Y"><LastName>Vivanco</LastName><ForeName>Jorge M</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Reardon</LastName><ForeName>Kenneth F</ForeName><Initials>KF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>02</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Bioeng</MedlineTA><NlmUniqueID>7502021</NlmUniqueID><ISSNLinking>0006-3592</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056804">Biofuels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D056804" MajorTopicYN="N">Biofuels</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017753" MajorTopicYN="Y">Ecosystem</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010568" MajorTopicYN="N" Type="Geographic">Peru</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>07</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>11</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>12</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>1</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>1</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23297115</ArticleId><ArticleId IdType="doi">10.1002/bit.24833</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Badri, Dayakar V" sort="Badri, Dayakar V" uniqKey="Badri D" first="Dayakar V" last="Badri">Dayakar V. Badri</name><name sortKey="Decker, Stephen R" sort="Decker, Stephen R" uniqKey="Decker S" first="Stephen R" last="Decker">Stephen R. Decker</name><name sortKey="Hunter, William J" sort="Hunter, William J" uniqKey="Hunter W" first="William J" last="Hunter">William J. Hunter</name><name sortKey="Manter, Daniel K" sort="Manter, Daniel K" uniqKey="Manter D" first="Daniel K" last="Manter">Daniel K. Manter</name><name sortKey="Reardon, Kenneth F" sort="Reardon, Kenneth F" uniqKey="Reardon K" first="Kenneth F" last="Reardon">Kenneth F. Reardon</name><name sortKey="Santhanam, Navaneetha" sort="Santhanam, Navaneetha" uniqKey="Santhanam N" first="Navaneetha" last="Santhanam">Navaneetha Santhanam</name><name sortKey="Vivanco, Jorge M" sort="Vivanco, Jorge M" uniqKey="Vivanco J" first="Jorge M" last="Vivanco">Jorge M. Vivanco</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Huang, Xing Feng" sort="Huang, Xing Feng" uniqKey="Huang X" first="Xing-Feng" last="Huang">Xing-Feng Huang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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